Figure 4.

TSP-1 caused dose and time-dependent increases in HPASMC Nox4 expression and ROS generation. Western blot to detect Nox4 protein was performed on protein isolated from HPASMC monolayers treated with (A) graded concentrations of TSP-1 for 72 hours (n = 3), or (B) 1 μg/ml of TSP-1 for 24-72 hours (n = 3). Nox4 immunoblots are presented below the bar graph. (C) HPASMC monolayers were treated with graded concentrations of TSP-1 for 72 hours and H₂O₂ production was measured by Amplex Red assay (n = 3). (A) ^*P< 0.01 vs. Control, (B) ^*P< 0.001 vs. Control, (C) ^*P< 0.01 vs. Control.