Figure 6.

TSP-1 induces Nox4 expression and rosiglitazone fails to attenuate this increase. HPASMC were treated with TSP-1 (1 μg/ml) for 72 hours and rosiglitazone (10 μM) was added to the cell culture media during the final 24 hours of treatment. Total RNA was collected and quantitative real-time PCR of Nox4 transcripts was performed. Each bar represents the mean ± SEM Nox4 transcripts normalized to 9S and expressed as fold change relative to control (n = 3-6). ^*P < 0.05 vs. Control.