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Characterization of a designed riboswitch module. A) Secondary-structure model of the proposed switchable RNA sequence 9. B) Chemical structure of tobramycin. C) Imino proton NMR spectra of RNA 9 (top) and after addition of 0.5 equivalents of tobramycin (bottom). D) Same as (C), but 19F NMR spectra for fold assignment. Conditions: [RNA]=0.3 mm, [Na2HAsO4]=10 mm, pH 7.0, H2O/D2O=9:1, 298 K; [tobramycin]=0.15 mm.
