Fig. 5.

LARGEdTM and LARGE2dTM have distinct pH optima for the Xyl-T and GlcA-T activities. Data from two independent experiments are shown as relative activity (%). The highest activity in the dataset was arbitrarily set at 100%. The Xyl-T and the GlcA-T assays were carried out using GlcA-β-MU and Xyl-α1,3-GlcA-β-MU as the acceptors, respectively. The buffers used were: Acetate for pH 4.0–5.5 (empty circles), MES for pH 5.5–6.5 (closed circles), MOPS for pH 6.5–8.0 (empty triangles) and Tris–HCl for pH 8.0–9.0 (closed triangles). The details of the conditions are presented in the Materials and methods section.