Figure 1.

Schematic representation, SEM, and fluorescence images of the investigated particles. (a) Dielectrophoretic responses of isotropic and anisotropic particles coated with fibronectin. (b) SEM images of plain PS (b1) and Janus particles (100 nm Au/1 μm PS) (b2). (c) Fluorescence images of 1 μm PS particle coated with fibronectin (adsorption (c1) or covalent coupling (c2)) and JP ((c3) green: fluorescence stained PS particle side, red: fibronectin on Au side). The difference is size between adsorbed (c1) and covalent coupling (c2), however, the same size, may be the difference in the scattered fluorescence intensity of those particles. The present work shows a much higher surface concentration of fluorescent fibrinogen in the adsorption coupling than the one in the covalent coupling. The scale bar indicates 1 μm.