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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Surgery. 2012 Aug;152(2):277–285. doi: 10.1016/j.surg.2012.05.006

Figure 3. Decreased phosphorylation of 4E-BP1 correlates with increased apoptosis.

Figure 3

(A) HCT116 and (B) DLD-1 cells were plated in 6 well plates at a density of 1.2×105 cells/well. Cells were transfected 12 h later with 100 nM NTC siRNA, 50 nM PIK3CA siRNA + 50 nM NTC siRNA, 50 nM KRAS siRNA + 50 nM NTC siRNA, or 50 nM PIK3CA siRNA + 50 nM KRAS siRNA. RNA was isolated 48 h post transfection and PIK3CA and KRAS mRNA levels measured using qRT-PCR (* p < 0.05 vs. control; RQ = relative quantity). HCT116 and DLD-1 cells were again plated and transfected under the same conditions to evaluate corresponding protein expression. Whole cell lysates were collected at 72 h and analyzed by Western blot (20 to 60 ug total protein per lane). β-actin was used as a loading control.