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Immunocytochemical confirmation of hESC and derivative lineages. Undifferentiated H1 hESCs (a) and MSC-like cells (b), and RCM-1 trophoblast-like cells ((c), (d)) stained for OCT4 (a), MSC lineage markers CD105 (red) and Stro-1 (green) (b) and trophoblast markers CDX2 (green, (c)) and hCG-β (green, (d)). Nuclei are counterstained with DAPI (blue) in all panels. Scale bar equals to 20 μm.
