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Skeletonization of microfibers in 3D. Orthogonal maximum projection images and associated skeletal representation of a microfiber in 3D. (A, B) 3D binary representation and accompanying skeleton of a raw 3D image set. (C, D) Deconvolved 3D data. (E, F) Deconvolved and Gaussian blurred data. Images are of a representative fiber from the 50 μm microfiber set.
