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Primary cilia lengths obtained from 2D and 3D measurement methods. (A) Chondrocyte primary cilia in vitro measured using 2D maximum projection images from confocal laser scanning microscopy (CLSM) data, the new 3D method and from 2D maximum projection (MP) images created from the 3D data. (B) Kidney primary cilia in situ, measured using 2D and 3D methods. In all instances the 3D measurement method produced a larger mean primary cilia length. (*P ≤ 0.05, **P ≤ 0.01).
