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Making the point spread function (PSF) spherical
| Raw | Deconvolved | Deconvolved and Gaussian blurred | |
|---|---|---|---|
| xy (nm) | 540 ± 76.4 | 324 ± 76.4 | 1,080 ± 76.4 |
| xz (nm) | 2,000 ± 176.8 | 1,000 ± 176.8 | 1,000 ± 176.8 |
| Ratio xy:xz | 0.27 ± 0.01 | 0.32 ± 0.02 | 1.08 ± 0.02 |
Full-width-half-maximum (FWHM) measurements of experimental PSFs for raw, deconvolved and deconvolved and Gaussian blurred 3D images (n = 5).
