Figure 4.

(a) Culture of MRC5 (left microchambers) and A549 (right microchamber) cells 24 h after seeding. (b) Culture of Balb/3T3 (left microchambers) and A549 (right microchamber) cells 24 h after seeding (the connecting microchannel with a length of 30 μm). Pictures show that all of the cultured cells are very well adhered to the growth surface. It indicates that the culture of two types of cells and medium exchanging along connecting microchannel not influence the viability and growth of these cells. Moreover, it was observed that 24 h after cells seeding, non-malignant cells grow in the shortest connecting microchannels. Type of the cells was distinguished based on their morphology. However, in the independent tests, cell trackers were used to mark two types of cells (see supplementary material⁴²).