Table 1. Primers used for the RT-qPCR validation design.

miRNA/Cluster	Forward primer (5′-3′)	Reverse primer (5′-3′)	Primer conc. (nM each)
Bta-miR-193b	AGAACTGGCCCACAAAGTC	TCCAGTTTTTTTTTTTTTTTAGCGG	250
Ssc-miR-423-5p	TGAGGGGCAGAGAGCGA	GTCCAGTTTTTTTTTTTTTTTAAAGTC	250
Ssc-miR-29c	GCAGTAGCACCATTTGAAATC	GGTCCAGTTTTTTTTTTTTTTTAACC	250
Ssc-miR-486	GCAGTCCTGTACTGAGCTG	GGTCCAGTTTTTTTTTTTTTTTCTCG	250
Ssc-let-7f	CGCAGTGAGGTAGTAGATTG	AGGTCCAGTTTTTTTTTTTTTTTAACT	250
Cl-2	CAGCTGCTATGCCAACA	CCAGTTTTTTTTTTTTTTTGGCAA	250
Cl-5	GCTGTAACAGCAACTCCA	TCCAGTTTTTTTTTTTTTTTCCAC	500
Cl-15	GCGACCCACTCTTGGT	TCCAGTTTTTTTTTTTTTTTCATGG	No amplification
Cl-16	CAGTTGGTGACCAGGTG	AGTTTTTTTTTTTTTTCTCCCTGA	No amplification
Cl-24	GCTGCATTTCCTGGCTG	GGTCCAGTTTTTTTTTTTTTTTAATTAAG	No amplification
Cl-25	CAGCTGGTGTTGTGAATC	GTTTTTTTTTTTTTTTCGGCCT	125
Cl-29	GGTTGGTGTACACTGGAA	GTCCAGTTTTTTTTTTTTTTTAGCT	250
Cl-38	GTCTCCGTTTGCCTGT	CCAGTTTTTTTTTTTTTTTCAGCA	250