Table 5. Selected miRNA and putative novel miRNAs (Clusters) for further validation through RT-qPCR.
miRNA/Cluster | Total counts | IsomiRs | Sequence (5′-3′) |
Hsa-miR-200b-3p | 27,097 | 123 | TAATACTGCCTGGTAATGATGA |
Ssc-miR-126 | 5,274 | 49 | TCGTACCGTGAGTAATAATGCG |
Ssc-miR-99a | 3,781 | 32 | AACCCGTAGATCCGATCTTGTGA |
Hsa-miR-200c-3p | 3,478 | 32 | TAATACTGCCGGGTAATGATGGA |
Ssc-miR-126* | 2,796 | 26 | CATTATTACTTTTGGTACGCG |
Ssc-miR-92a | 797 | 17 | TATTGCACTTGTCCCGGCCTGT |
Ssc-miR-26a | 598 | 17 | TTCAAGTAATCCAGGATAGGCT |
Bta-miR-193b | 493 | 26 | AACTGGCCCACAAAGTCCCGCT |
Ssc-miR-532-5p | 113 | 3 | CATGCCTTGAGTGTAGGACCGT |
Ssc-miR-423-5p | 74 | 10 | TGAGGGGCAGAGAGCGAGACTTT |
Ssc-miR-29c | 23 | 3 | TAGCACCATTTGAAATCGGTTA |
Ssc-miR-486 | 23 | 4 | TCCTGTACTGAGCTGCCCCGAG |
Ssc-let-7f | 17 | 1 | TGAGGTAGTAGATTGTATAGTT |
Cl-2 (Hsa-miR-31-3p)1 | 4 | 1 | CTGCTATGCCAACATATTGCCA |
Cl-5 (Hsa-miR-194-5p)1 | 12 | 2 | CTGTAACAGCAACTCCATGTGGAA |
Cl-15 (Hsa-miR-551a)1 | 9 | 2 | GCGACCCACTCTTGGTTTCCATG |
Cl-16 | 6 | 1 | TTGGTGACCAGGTGCTCAGGGAG |
Cl-24 | 3 | 2 | CTGCATTTCCTGGCTGCCTTAATT |
Cl-25 (Hsa-miR-138-5p)1 | 7 | 3 | CAGCTGGTGTTGTGAATCAGGCCG |
Cl-29 | 3 | 1 | GTTGGTGTACACTGGAATAGCT |
Cl-38 (Bta-miR-1468)1 | 21 | 3 | TCTCCGTTTGCCTGTTTTGCTGA |
: miRBase (v18) homology (e-value ≤1e-03). Internal mismatches were accepted.
Bta: Bos taurus, Hsa: Homo sapiens, Ssc: Sus scrofa.