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. 2013 Jan 25;8(1):e55202. doi: 10.1371/journal.pone.0055202

Figure 2. Basic phenotyping of SGEF deficient mice.

Figure 2

A: RT-PCR from mouse liver mRNA using primers which bind in exon 1 and in different downstream exons of mouse SGEF as indicated. RT-PCR bands from SGEF−/− animals marked with an asterisk are ∼200 bp smaller compared to wild type. B and C: SGEF ex1-14 cDNA cloned from RT-PCRs from SGEF+/+ and SGEF−/− mice was over expressed in HeLa cells. The transfected HeLa cells were analyzed for dorsal ruffle formation (arrowheads) (B) and the activity levels of RhoG (C). Graph at the right shows fold increase of Rho.GTP loading upon transfection or treatment as indicated. This experiment was repeated 4 times. D: Expression of SGEF in different mouse tissues. The polyclonal antibody used was raised against a 200 amino acid stretch at the N-terminus of murine SGEF.