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. 2013 Jan 25;8(1):e55202. doi: 10.1371/journal.pone.0055202

Figure 4. Reduced atherosclerotic plaque formation in SGEF deficient mice fed a Western diet.

Figure 4

SGEF+/+ApoE−/− and SGEF−/−ApoE−/− mice were fed a Western Diet for 14 weeks post-weaning (weaning at 3 weeks of age), before aortas were isolated and the plaque area was determined by ORO staining. A: Representative en face images of ORO staining of the inner curvature of the aortic arches. B: Quantification of ORO-stained area on inner curvature of the aortic arches from SGEF+/+ApoE−/− and SGEF−/−ApoE−/− (n = 10 per genotype; SGEF+/+ApoE−/−: 15.8±3.7%; SGEF−/−ApoE−/−: 8.8±3.7%; Data are mean ± SD. ***p<0.006. Bar graph at the right represents averaged measurements of all studied aortic arches. Left graph shows the measured ORO-stained area of individual aortas (represented by diamonds). C and D: As described under A and B, but for the descending thoracic aorta: D: Quantification of plaque area in the: SGEF+/+ApoE−/−: 3.2±1.9%; SGEF−/−ApoE−/−: 1.1±0.5%; Data are mean ± SD. ***p<0.007. E: Histology samples were stained for CD68 or ICAM-1 as indicated to detect macrophage presence in plaque areas. Graph at the right shows the quantification of CD68 positive areas in the plaque. n = 4. Asterisks indicate *p<0.05 according to Student T-test.