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. 2013 Jan 25;8(1):e54472. doi: 10.1371/journal.pone.0054472

Figure 7. IRF7 binds to the an IRF7-RE located in the FXR promoter.

Figure 7

(A) Electrophoretic Mobility shift assay (EMSA). Nuclear extracts from Raw264.7 cells left untreated or stimulated with CpG were incubated in the presence of a wild type or a mutated IRF7 biotin-labeled probe. Competition experiments were performed with a 100 fold excess of unlabeled oligo or with 1 µg IRF7 antibody. (B) Chromatin immunoprecipitation (ChIP). ChIP assay carried out in Raw264.7 cells left untreated or primed with CpG as described in materials and methods section. Values are normalized relative to input DNA concentration and are expressed relative to those of not treated cells immunoprecipitated with an anti IgG antibody, condition set as 1. Analysis was carried out in triplicate and the experiment was repeated twice. *P<0.05 versus not treated cells immunoprecipitated with an anti-IgG antibody. #P<0.05 versus not treated cells immunoprecipitated with an anti-IRF7 antibody.