Table 1. Mutant nucleotide and amino acid changes of the recombinant virus, rA/P1-FMDV, obtained in BHK–21 cells at passages 5, 13, 22, 26 and 30.
| Plasmid nameor passage time | Mutant nucleotide and amino acid changes related to prA/P1-FMDVa | |||||||||||
| 5′UTRb | L | VP2 | VP3 | VP3 | VP1 | 2C | 3A | 3A | 3D | |||
| 261c | 701 | 995 | 1238 | 2230 | 2637 | 2827 | 3628 | 5256 | 5517 | 5694 | 7056 | |
| prA/P1-FMDV | T | A | T | A(Thrd) | A(Lys) | A(Pro) | A(Glu) | C(His) | T(Val) | A(Gln) | C(Asp) | C(Leu) |
| 5e | T | A | T | A(Ser) | A(Lys) | A(Pro) | A(Glu) | A(Gln) | C(Val) | A(Gln) | T(Asp) | T(Leu) |
| 13 | G | A | C | A(Ser) | G(Lys) | G(Pro) | G(Glu) | A(Gln) | T(Val) | A(Gln) | T(Asp) | T(Leu) |
| 22 | G | T | C | A(Ser) | A(Lys) | G(Pro) | A(Glu) | A(Gln) | C(Val) | A(Gln) | T(Asp) | T(Leu) |
| 26 | G | A | C | A(Ser) | A(Lys) | G(Pro) | A(Glu) | A(Gln) | C(Val) | A(Gln) | T(Asp) | T(Leu) |
| 30 | G | A | C | A(Ser) | A(Lys) | G(Pro) | A(Glu) | A(Gln) | C(Val) | G(Gln) | T(Asp) | T(Leu) |
a
Full-length genome sequencing of five viruses was performed and compared to plasmid prA/P1-FMDV.
b
Virus region.
e
Nucleotide position.
d
Corresponds to amino acid or amino acid substitution.
e
Passage time.