Figure 5.

Effect of carboplatin and paclitaxel on TOV112D spheroids. The reference IC₅₀ is based on drug responses in TOV112D monolayer cultures. (a) Merged CTG-PI confocal section (image layer #13) of spheroids subjected to carboplatin and paclitaxel at different concentrations. No image is provided for the 100 × IC₅₀ paclitaxel treated spheroids, as under these conditions they lost their structural integrity and were fragmented during the vital dye staining. (b) Variation in the distribution of mortality fractions across all z-sections with varying carboplatin concentrations (1, 10, and 100 × IC₅₀). (c)Variation in mortality distribution with changes in paclitaxel concentration (1 and 10 × IC₅₀). Treatment time is 24 h for all samples. Each data point is averaged over three independent experiments (with total number of samples, n = 16) and shown as mean ± standard deviation.