Figure 1. NK cell line growth inhibition and apoptosis induced by resveratrol.

Cells were plated in triplicate and treated with increasing concentrations of resveratrol (3.125, 6.25, 12.5, 25 and 50 µM) for 48 h (A) or 50 µM for 24, 48 and 72 h (B). Cell proliferation was then evaluated using the MTT assay. For the apoptosis study, cells (1×10⁶/ml) at 37°C were treated with resveratrol at the indicated concentrations for 24 h (C) or with 50 µM of resveratrol for the indicated times (D). The cells were then incubated with anti-annexin V antibody conjugated to FITC and analysed by flow cytometry. Data represent the drug-induced increase in the percentage of apoptotic cells compared to the respective values observed in parallel control cultures. Results are the mean of data, with error bars representing the SD of triplicate values. (E) Cells were treated with 50 µM of resveratrol for 12 and 24 h and stained with Giemsa to observe morphological changes. The images shown are representative results of three independent experiments.