Figure 5. Effect of resveratrol on the p21/p53 axis and Mdm2/p53 axis in NK cell lines.

(A) Cells were treated with 50 µM of resveratrol for 24, 36 and 48 h. 50 µg of whole cell extract was prepared for each cell line, separated by SDS-PAGE and subjected to Western blotting with antibodies specific to Mdm2, p53 and p21 antibodies. The blots were stripped and reprobed with anti- α-tubulin antibody to show equal protein loading. (B) Cells (1×10⁶/ml) were incubated with pifithrin α for 2 h in one group before treatment of 50 µM of resveratrol for 24 h. The cells were then incubated with anti-annexin V antibody conjugated to FITC and analysed by flow cytometry to evaluate apoptosis. The figures shown are representative results of three independent experiments.