Figure 1. Viral vectors used in this study (A) and their titers (B).

(A) A schematic representation of the engineered viruses used in this study. All viruses are based on E1-delated human Ad5. The transgenes (GFP or pp65) are under control of the human cytomegalovirus (CMV) immediate early promoter. The sequence encoding Tat-PTD was inserted into the hexon HVR5 region. The gene encoding the adenovirus fiber shaft and knob was either kept from Ad5 or replaced with the Ad35 counterparts. (B) Titers of the viruses used in this study established by three methods of determination: encapsidated virus genomes (evg) based on quantitative PCR, fluorescent forming units (FFU) based on infection of 911 cells and virus particles (vp) based on optical density measurements.