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. 2013 Jan 25;8(1):e54968. doi: 10.1371/journal.pone.0054968

Figure 4. Role of miR130b on the PPARα/HIF1α interplay in breast CSCs.

Figure 4

(A) miR130b qPCR analysis in TAF supernatant (10%, 24 h)-exposed MCF7-MS; (B) DDX6 qPCR analysis in TAF supernatant (10%, 24 h)-exposed MCF10/MCF7-MS and N−/T-MS (samples, 14 and 15); HRELuc assay (C) and DDX6 qPCR mRNA analysis (D) in AntagomiR130b (a-miR130b, 48 h)-transfected MCF7-MS; (E) miR130b qPCR analysis in MCF7 and MCF10 cultured as adherent or MS; (F) miR130b qPCR analysis in SCR/siHIF1/siPPARα (72 h) and pV/pPPARα vector (24 h)-transfected MCF7-MS; (G) PPARα qPCR analysis in pre-miR130b (48 h)-transfected MCF7-MS. Data are expressed as mean ±S.D., n = 3, *p<0.05, # p<0.01, § p<0.005, ANOVA test. n.s.: not significant.