Table 4.
The Cauda epididymal sperm characteristics and Preimplantation loss postimplantation loss and antifertility activity in rats control and treated orally with the Methanolic extract of the bark of Aegle marmelos (L.) 200 mg, 400 mg and 600 mg per kg body weight (bw) per day (values are mean ± SEM, n = six animals)
| Treatment schedule | Sperm density (million/mL) | Acrosomal integrity (%) | Sperm viability (%) | Sperm motility (%) | Pre implantation loss (%) | Post implantation loss (%) | Anti-fertility (%) | |
|---|---|---|---|---|---|---|---|---|
| C |
|
39.47 ± 3.87 |
73.54 ± 7.21 |
67.83 ±5.67 |
77.17 ±7.60 |
14.36 ± 2.41 |
2.57 ± 1.60 |
18.32 ± 2.74 |
| SD |
20 D |
28.92 ± 2.71 a |
57.17 ± 5.61 a |
56.67 ±4.74 a |
59.33 ± 6.01 a |
42.71 ± 6.41 a |
12.22 ± 2.94 a |
54.74 ± 8.42 a |
| |
40 D |
21.27 ± 2.09 a |
49.00 ± 4.70 a |
46.07 ±3.63 a |
27.54 ± 2.84 a |
60.05 ± 9.04 a |
6.43 ± 0.79 a |
66.48 ± 9.64 a |
| |
60 D |
16.77 ±1.79 a |
38.17 ± 3.71 a |
27.04 ± 3.01 a |
NIL |
91.21 ± 14.14 a |
8.79 ± 1.1 a |
100.0 ± 0 a |
| G I |
20 D |
34.96 ±3.40 |
64.83 ± 6.40 a |
61.17 ± 5.20 a |
61.33 ±6.09 a |
23.32 ± 3.84 a |
18.22 ± 3.20 a |
41.54 ± 6.17 a |
| |
40 D |
28.65 ±2.76 a |
56.67 ± 5.59 a |
54.17 ±4.34 a |
30.17 ±3.05 a |
45.57 ± 6.71 a |
14.62 ± 3.07 a |
60.19 ± 9.01 a |
| |
60 D |
21.48 ±2.10 a |
41.59 ± 4.10 a |
44.17 ±3.57 a |
3.67 ±3.71 a |
100.0 ± 0 a |
NA |
100.0 ± 0 a |
| G II |
20 D |
26.22 ±2.51 a |
52.17 ± 5.21 a |
52.00 ±4.20 a |
50.83 ± 5.09 a |
41.35 ± 6.21 a |
16.89 ± 3.14 a |
58.23 ± 8.76 a |
| |
40 D |
20.92 ±1.98 a |
41.17 ± 4.09 a |
45.56 ±3.61 a |
27.33 ± 2.81 a |
63.03 ± 9.18 a |
21.94 ± 3.71 a |
84.97 ± 11.43 a |
| |
60 D |
16.88 ±1.54 a |
16.17 ± 1.71 a |
38.67 ±3.12 a |
Nil |
100.0 ± 0 a |
NA |
100.0 ± 0 a |
| G III |
20 D |
26.0 ±2.51 a |
40.67 ± 4.01 a |
48.33 ±3.84 a |
38.50 ±3.91 a |
57.38 ± 8.79 a |
6.91 ± 2.19 a |
64.34 ± 9.45 a |
| |
40 D |
17.05 ±1.67 a |
15.07 ± 1.54 a |
34.83 ±2.97 a |
Nil |
100.0 ± 0 a |
NA |
100.0 ± 0 a |
| |
60 D |
12.33 ±1.27 a |
9.833 ± 0.94 a |
21.17 ±2.21 a |
Nil |
100.0 ± 0 a |
NA |
100.0 ± 0 a |
| RG I |
30 D |
37.14 ±3.67 |
62.83 ± 6.21 a |
63.0 ±5.50 |
72.33 ±7.34 |
16.25 ± 2.74 |
2.57 ± 1.59 |
18.82 ± 2.87 |
| RG II |
30 D |
35.52 ±3.44 |
55.00 ± 5.49 a |
63.83 ±5.67 |
64.83 ±6.50 |
19.77 ± 1.57 |
2.21 ± 1.40 |
21.99 ± 3.90 |
| RG III | 30 D | 30.33 ±2.97 | 49.67 ± 4.87 a | 57.17 ±4.74 | 63.83 ±6.44 | 23.14 ± 3.77 | 2.89 ± 1.71 | 26.04 ± 4.10 |
Significance level: a P ≤ 0.01 compared Control.
C- Rats treated with treated with in 2.0 ml 0.5 percent CMC each day for 60 days.
G I- Rats treated with methanolic bark extract of Aegle marmelos 200 mg/rat/day/p.o.
GII- Rats treated with methanolic bark extract of Aegle marmelos 400 mg/rat/day/p.o.
GIII- Rats treated with methanolic bark extract of Aegle marmelos 600 mg/rat/day/p.o.
RG-I- Rats withdraw from treated for 30 days after 60 days treatment of methanolic bark extract of Aegle marmelos 200 mg/rat/day/p.o.
RG-II- Rats withdraw from treated for 30 days after 60 days treatment of methanolic bark extract of Aegle marmelos 400 mg/rat/day/p.o.
RG-III- Rats withdraw from treated for 30 days after 60 days treatment of methanolic bark extract of Aegle marmelos 600 mg/rat/day/p.o.