Table 3.
GenBank accession numbers, PCR primers, amplicon sizes and PCR efficiencies of the RT-qPCR assays used in the current experiment
| Gene | Accession no. | Forward primer | Reverse primer | Amplicon size(bp) | PCR efficiency |
|---|---|---|---|---|---|
| CYP1A |
EX725014 |
CCTTGACCTCTCGGAGAAAGAC |
CGCCCCGCTAGCTATAGACA |
146 |
1.85 |
| CYP1B1 |
GmE090818c16636 |
CAGATGCCCCACATCACCTT |
GATCGCCTTGTCTCCGTTCA |
108 |
2.10 |
| CYP1C1 |
GmE100215i31189 |
GTCAACCAGTCGTCCGTGAA |
CGTTGTTCGTGAGGTCCTTGT |
115 |
2.02 |
| AhR2 |
EX728781 |
CAACCGGCGGTCCACAT |
GCACCATGCAGTTGCCAGTA |
132 |
2.08 |
| AHRR |
F2Z8C0H01CDEIU |
GAGGTGTTTGTCTCCCGTCTCT |
TTCACCAGGTCCACGCAGTA |
77 |
2.06 |
| GST pi |
EX730032 |
GTCCCCCTGCTGCCATTC |
CCTCCATACACCGCCACCTA |
126 |
1.85 |
| p53 |
EX723548 |
CGCTGCTGCTGAACTTCATG |
GGATGGCTCTCCGGTTCAT |
63 |
1.86 |
| RAD51 |
|
AACCTAGCATCCCGCTCAAA |
TGGTCAGGACAACTGGGATGT |
91 |
1.89 |
| IGF1 |
|
CTCTCCAAATCCGTCTCCTGTT |
AGATGCGGGCAATGTCACAT |
101 |
1.99 |
| IGFBP1 |
|
GTGAGTCCTCCCTGGATGGA |
CTCGACCCCAGGATCTTCTTC |
79 |
2.02 |
| Ferritin |
EX726260 |
GCGTCTAGCTGGACCCCTTA |
GGGCGACTACATCAGCAACC |
101 |
2.08 |
| Transferrin |
EX722617 |
ACGACGGGTCAACTCGTTTG |
AGTCACTCGGGCACAATGTG |
139 |
1.83 |
| HSP70 |
EX741726 |
CATGACATCGTCCTGGTTGGT |
CGTAGGCCACAGCTTCATCA |
121 |
1.84 |
| HPX (WAP65-2) |
GmE100215i27905 |
CGCCTGCTCTACATCGTCAA |
CGTAGCGTCCAGTGGTCCTT |
129 |
1.89 |
| MCM2 |
GmE100215i14387 |
TGGAGGACGACGTGAACATG |
TCGTTGTTGTCGCGTTTGAA |
130 |
1.77 |
| CDCA7 |
GmE100215i27789 |
GCATCTGCACCAACGTGAAG |
CCCCCACACACTCTGGGTTA |
120 |
2.02 |
| EF1A |
EX722124 |
CGGTATCCTCAAGCCCAACA |
GTCAGAGACTCGTGGTGCATCT |
93 |
1.97 |
| B-actin |
EX739174 |
CACAGCCGAGCGTGAGATT |
ACGAGCTAGAAGCGGTTTGC |
95 |
1.93 |
| Ubiquitin |
EX735613 |
GGCCGCAAAGATGCAGAT |
CTGGGCTCGACCTCAAGAGT |
69 |
1.93 |
| RPL4 | EX725958 | GGTGCCATACAGCTGATCCA | CCAGGCATCACACTGCAGAA | 123 | 1.83 |