Table 3.
Species-specific results among malaria genus positive samples
Samples (n = 28) | Sequencing PgMt19 F3 & B3 [21] | Modified species-specific 18S PCR [26] | Species-specific nested 18S PCR [10] | Microscopy |
---|---|---|---|---|
18 |
Pf |
Pf |
Pf |
Pf |
1 |
Pf |
Pf + Pm |
Pf + Pm |
Pf |
1 |
Pf |
Negative |
Negative |
Pf |
3 |
Pv |
Pv |
Pv |
Pv |
1 |
Pv |
Pv |
Pv |
Negative |
1 |
Pv |
Pv |
Pv |
Pf + Pv |
1 |
Pv |
Pv1 |
Pv1 |
Pk2 |
1 |
Po |
Po |
Po |
Pf + Po |
1 | Pm | Pm | Pm | Negative |
Abbreviations: Pf, Plasmodium falciparum; Pv, Plasmodium vivax; Po, Plasmodium ovale; Pm, Plasmodium malariae; Pk, Plasmodium knowlesi.
1Plasmodium knowlesi primers were not included in the assay.
2 Evaluated as inconclusive species by microscopy, but with emphasis on possible Plasmodium knowlesi infection.