Figure 2.

Pho7 is recruited to phosphate-regulated promoters during Pi starvation. Cells lacking the TAP epitope tag (Mock) or containing an epitope fused version of Pho7 (Pho7-TAP) were assayed for their enrichment of Pho7 at the pho1⁺ promoter via ChIP-Seq. Cells were either incubated in high-Pi conditions (blue) or starved (red) for 2 hours before cross-linking. Reads were normalized to total counts for each chromosome. Magnified ChIP-Seq profiles for pho1⁺, SPBC8E4.01c, SPBC1271.09, SPAC9E9.09c, SPBPB2B.05, and SPCC330.06c are shown. Mock samples lacking the TAP epitope (black) were treated identically to experimental samples. Coordinates are relative to the initial ATG, with gene products identified by black bars.