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. 2012 Dec 5;10:104. doi: 10.1186/1477-7827-10-104

Figure 2.

Figure 2

Electrophoretic Mobility Shift Assays (EMSA) for testicular HIF-1 binding. The DNA-binding activity of testicular HIF-1 was determined in EMSA experiments. (A) Results of positive control experiment using biotin-labelled oligonucleotides for Epstein-Barr nuclear antigen (EBNA), a positive control protein extract for EBNA (Ext), competition with unlabeled EBNA oligonucleotides, and competition with non-specific oligonucleotides for β-actin. (B) Representative EMSA results for cytoplasmic or nuclear (C) protein extracts from normoxic testes incubated with biotin-labelled HIF-1 oligonucleotides containing the consensus HRE. Actin lanes contain a molar excess of unlabeled β-actin oligonucleotides to demonstrate the specificity of protein binding to the HIF-1 oligonucleotides.