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EMSA supershift and competition assays for testicular HIF-1 binding. (A) EMSA results when using cytoplasmic extracts of testicular HIF-1 and the resulting supershifts demonstrated with C19 and Y15 polyclonal antibodies specific for HIF-1α. (B) Competition experiments to determine HIF-1 EMSA binding specificity used unlabeled HIF-1 HRE consensus oligonucleotides at increasing concentrations to abrogate HIF-1 oligonucleotide binding by testicular extracts.
