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HIF-1 from the normoxic and ischemic testes binds the HIF-1 consensus HRE. Cytoplasmic and nuclear extracts of testicular proteins from normoxic sham-treated (S) and 1 hour ischemic (I) testes were subjected to EMSA to demonstrate HIF-1 binding. Testicular HIF-1 from ischemic and sham-treated testes bound DNA equally.
