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. 2012 Dec 5;10:104. doi: 10.1186/1477-7827-10-104

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Copyright ©2012 Palladino et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Mcl-1 is abundantly expressed in the testis, and Mcl-1 levels do not change following ischemia or ischemia-reperfusion. (A) Results of Mcl-1 immunoblot analysis of nuclear proteins isolated from experimentally-induced unilaterally ischemic testes (I) and sham-treated testes (S). Aliquots containing 75-μg of nuclear proteins were analyzed from testes subjected to 1 h or 6 h of ischemia, and ischemia and reperfusion (I/R) for 1h/4h. Mcl-1 was detected at ~40kDa. Extracts from the PC-12, Ramos and BJAB cell lines were used as positive controls for detecting Mcl-1. Activating transcription factor-2 (ATF-2) was detected as a loading control as a protein that is constitutively expressed and is unaffected by ischemia. (B) Histogram showing Mcl-1 quantitation normalized to ATF-2 as a percentage of the sham controls. No statistically significant differences in the steady state levels of the Mcl-1 protein were observed between the ischemic and normoxic groups (ANOVA, p<0.05, n=3-5).