Table 3.

Primers used in this study

Primer Name	Sequence (5′-3′)	Used for plasmid
gldASpeIfwd	aggatcactagtaggagaagttaccatggaccgcattattcaatcaccgg	GYM
gldA_YqhDrev	ggtgtgcagattaaagttgttcatggtaacttctcctttattcccactcttgcaggaaac	GYM
gldA_YqhDfwd	gtttcctgcaagagtgggaataaaggagaagttaccatgaacaactttaatctgcacacc	GYM
YqhD_mgsArev	aaagtgcgagtcgtcagttccatggtaacttctcctttagcgggcggcttcgtatatacg	GYM
YqhD_mgsAfwd	cgtatatacgaagccgcccgctaaaggagaagttaccatggaactgacgactcgcacttt	GYM
mgsANotI rev	ggatcggcggccgcttacttcagacggtccgcgagataacgctga	GYM
CBSADH SpeIfwd	aggatcactagtaggagaagttaccatgaaagggtttgccatgttag	CYM
CBSADH_yqhDrev	gcagattaaagttgttcatggtaacttctcctttacaggataacaaccgc	CYM
CBSADH_yqhDfwd	gcggttgttatcctgtaaaggagaagttaccatgaacaactttaatctgc	CYM
TBSADH SpeIfwd	aggatcactagtaggagaagttaccatgaaaggttttgcaatgctgtc	TYM
TBSADH_yqhDrev	gcagattaaagttgttcatggtaacttctcctctatgctaaaatcaccac	TYM
TBSADH_yqhDfwd	gtggtgattttagcatagaggagaagttaccatgaacaactttaatctgc	TYM
pQE XhoI fwd	ttcacctcgagaaatcataaaaaatttatttgc	pZElac_his
pQE Acc65I rev	gttttcggatccgtgatggtgatggtgatgcgatcctctc	pZElac_his
his ad up rev	ggatccgtgatggtgatggtgatgcgatcc	his-gldA/CB/TB
his ad down fwd	tctagaggcatcaaataaaacgaaaggctc	his-gldA/CB/TB
his ad up_gldA fwd	gcatcaccatcaccatcacggatccatggaccgcattattcaatcaccgg	his-gldA
his ad down_gldA rev	cctttcgttttatttgatgcctctagattattcccactcttgcaggaaac	his-gldA
his ad up_CB fwd	atcaccatcaccatcacggatccatgaaagggtttgccatgttaggtatc	his-CB
his ad down_CB rev	tttcgttttatttgatgcctctagattacaggataacaaccgccttaatc	his-CB
his ad up_TB fwd	cgcatcaccatcaccatcacggatccatgaaaggttttgcaatgctgtcc	his-TB
his ad down_TB rev	ctttcgttttatttgatgcctctagactatgctaaaatcaccactggtt	his-TB
rnpBfwd	aggtgttggctcggtaaac	RT-PCR rnpB
rnpB rev	cgaagacagagggcagttatc
gldAfwd	gaaaccgtagctgcccttag	RT-PCR gldA
gldA rev	ggcatgttgtgaatggtttc
mgsAfwd	tgacgactcgcactttacct	RT-PCR mgsA
mgsA rev	cgtgttgttccagtaacggt
yqhDfwd	cgaacaaattcctcacgatg	RT-PCR yqhD
yqhD rev	tcatcagcgtttcataagcc
CB fwd	ctccagtgtggtggttatcg	RT-PCR CBsADH
CB rev	ttagctgcttcaacgcaaat
TB fwd	tatggtgctaccgacatcgt	RT-PCR TBsADH
TB rev	taattgacgtttgcgatggt