Figure 5.

Candidate loci with interindividual variation. DNA from 17 isogenic mouse livers at d21 was isolated and bisulfite converted. Amplification and digestion of 21 representative IAP elements show variable levels of methylation per locus and mouse. Upper bands indicate uncut PCR product corresponding to the unmethylated BceAI site. Lower bands indicate a fully methylated BceAI site consisting of CpGs 2 and 3 from Figure 4. Locus 281Y is located on the Y chromosome and is therefore amplified only from male animals.