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. Author manuscript; available in PMC: 2014 Jan 18.
Published in final edited form as: Oncogene. 2012 Sep 17;32(29):3461–3469. doi: 10.1038/onc.2012.426

Figure 1. p68 is critical for DNA-damage induced G1/S cell cycle arrest and p21 expression but is not required for induction of pro-apoptotic genes.

Figure 1

MCF-7 cells were transfected with non-specific (NS), p68 or p21 siRNAs and treated with etoposide prior to analysis by flow cytometry or RNA extraction for qRT-PCR (TaqMan). Untreated cells served as controls. +: cells treated with 5μM etoposide for 16 h.

A: Percentage of cells in G1, S and G2 as determined by flow cytometry after BrdU treatment and propidium iodide staining. B-E: Levels of mRNAs as indicated shown relative to respective mRNA cells transfected with a non-specific siRNA and not treated with etoposide. In each case this is shown as 1. F: corresponding western blots.

In all graphs the values shown are the averages from 3 independent experiments +/− s.e.m. and statistically significant differences are indicated. Where graphs are marked by * instead of bars for clarity, p values were <0.01.