(A) Peptide sequence of phoenixin. Potential cleavage sites are indicated by red lettering on the consensus (top) sequence. Phoenixin is highly conserved across species.
(B) Phoenixin immunoreactivity can be detected in various tissues in adult rats, as indicated by EIA. Mass spectrometry analysis of endogenous phoenixin isolated from rat hypothalamus (C) and bovine heart (D) revealed peptides of 20 and 14 amino acids, respectively.