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. Author manuscript; available in PMC: 2014 Jan 21.
Published in final edited form as: Curr Biol. 2012 Dec 27;23(2):162–167. doi: 10.1016/j.cub.2012.11.049

Figure 3. siRNA knockdown of Med31 and Elmo1 decreases transcription of specific genes in BMDM after Salmonella infection.

Figure 3

A. qRT-PCR analysis of knockdown efficiency in BMDMs transfected with siRNA targeting Elmo1 (E1) and/or Med31 (M31). Total siRNA transfected in each sample was kept constant by addition of control (Ctrl) siRNA. B. Analysis of Il33 and Il10 transcript levels from siRNA-transfected BMDMs, either 6hr post Salmonella (ΔinvG) infection (grey bars) or uninfected (white bars), demonstrating a statistically significant decrease in transcript levels with knockdown of Elmo1 or Med31. C. siRNA knockdown of Elmo1 or Med31 in BMDM cells in response to Salmonella (ΔinvG) infection does not affect other transcripts with three examples shown here: a gene that was upregulated post infection (Tnf), one transcript unchanged with infection (Tgfb1), and one transcript downregulated after infection (Nfatc1). All transcript levels were normalized to the reference gene Hprt1. Data are represented as mean +/− standard deviation. Data for other transcripts analyzed is shown in Supplemental Table S2.