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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Exp Eye Res. 2012 Dec 8;107:121–129. doi: 10.1016/j.exer.2012.11.004

Figure 5.

Figure 5

Figure 5

TRPV1 gene silencing reduces CAP-induced MAPK activation. (A) Validation of TRPV1 gene knockdown by siRNA. Western blot analysis compares TRPV1 protein expression level in cells transfected with either scrambled siRNA or TRPV1 siRNA. Housekeeping gene GAPDH confirmed equivalent protein loading. (B) TRPV1 gene silencing reduced CAP (20 μM) induced ERK1/2, JNK/SAPK and p38 MAPK phosphorylation at 15 min. GAPDH was used as loading controls. Representative results obtained from three independent experiments are shown.