Fig. 7.

A working model for CM differentiation in our 2D-direct differentiation system. Undifferentiated hPSCs were expanded at stage 1. Meso-endoderm lineage cells were induced from the peripheral region of undifferentiated hPSCs at stage 2. Mesoderm lineage cells were committed to cardiac lineage cells in aggregated clusters and endoderm cells were localized to the periphery of aggregated clusters at stage 3. Contracting clusters were further differentiated into cardiomyocytes at stage 4.