Figure 4.

NE facilitates engulfment of TC-1 cells by JAWS II cells. JAWS II cells were stained with CFSE (green fluorescence); TC-1 cells were stained with PKH-26 (red fluorescence). TC-1 cells were then incubated overnight with either NE (C, D) or staurosporine (E, F) or were left untreated (A, B). The next day, TC-1 ECs were mixed in equal number with CFSE-stained JAWS II DCs. The JAWS II cells were pretreated with 35 mM NaN₃ overnight (G). Mixed cultures were incubated for four hours either at 37°C or 4°C and analyzed using flow cytometry. Confocal microscopy microphotograph of TC-1 cells treated with NE and co-cultured with JAWS II cells (H). Data shown are representative of one of five independent experiments. Significant difference (p<0.05) in the percentage of engulfed TC-1 cells between untreated and treated with 0.05% of W₈₀5EC (N=5) has been observed as tested using Wilcoxon signed-rank test.