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. 1974 Nov;14(5):1099–1103. doi: 10.1128/jvi.14.5.1099-1103.1974

Nature of the Scrapie Agent: Evidence Against a Viroid

Richard L Ward 1, David D Porter 1, Jack G Stevens 1
PMCID: PMC355625  PMID: 4372389

Abstract

The postulated viroid nature of scrapie agent was tested. Since the agent could not be recovered after a carefully controlled phenol extraction of infected mouse brains, it is suggested that this agent is not a viroid.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alper T., Haig D. A., Clarke M. C. The exceptionally small size of the scrapie agent. Biochem Biophys Res Commun. 1966 Feb 3;22(3):278–284. doi: 10.1016/0006-291x(66)90478-5. [DOI] [PubMed] [Google Scholar]
  2. CHANDLER R. L. Encephalopathy in mice produced by inoculation with scrapie brain material. Lancet. 1961 Jun 24;1(7191):1378–1379. doi: 10.1016/s0140-6736(61)92008-6. [DOI] [PubMed] [Google Scholar]
  3. Diener T. O. Is the scrapie agent a viroid? Nat New Biol. 1972 Feb 16;235(59):218–219. doi: 10.1038/newbio235218a0. [DOI] [PubMed] [Google Scholar]
  4. Diener T. O. Potato spindle tuber "virus". IV. A replicating, low molecular weight RNA. Virology. 1971 Aug;45(2):411–428. doi: 10.1016/0042-6822(71)90342-4. [DOI] [PubMed] [Google Scholar]
  5. Diener T. O., Raymer W. B. Potato spindle tuber virus: a plant virus with properties of a free nucleic acid. II. Characterization and partial purification. Virology. 1969 Mar;37(3):351–366. doi: 10.1016/0042-6822(69)90219-0. [DOI] [PubMed] [Google Scholar]
  6. Diener T. O., Raymer W. B. Potato spindle tuber virus: a plant virus with properties of a free nucleic acid. Science. 1967 Oct 20;158(3799):378–381. doi: 10.1126/science.158.3799.378. [DOI] [PubMed] [Google Scholar]
  7. Gibbons R. A., Hunter G. D. Nature of the scrapie agent. Nature. 1967 Sep 2;215(5105):1041–1043. doi: 10.1038/2151041a0. [DOI] [PubMed] [Google Scholar]
  8. Griffith J. S. Self-replication and scrapie. Nature. 1967 Sep 2;215(5105):1043–1044. doi: 10.1038/2151043a0. [DOI] [PubMed] [Google Scholar]
  9. Hunter G. D., Gibbons R. A., Kimberlin R. H., Millson G. C. Further studies of the infectivity and stability of extracts and homogenates derived from scrapie affected mouse brains. J Comp Pathol. 1969 Jan;79(1):101–108. doi: 10.1016/0021-9975(69)90033-4. [DOI] [PubMed] [Google Scholar]
  10. Hunter G. D., Kimberlin R. H., Gibbons R. A. Scrapie: a modified membrane hypothesis. J Theor Biol. 1968 Sep;20(3):355–357. doi: 10.1016/0022-5193(68)90135-5. [DOI] [PubMed] [Google Scholar]
  11. Hunter G. D., Millson G. C. Attempts to release the scrapie agent from tissue debris. J Comp Pathol. 1967 Jul;77(3):301–307. doi: 10.1016/0021-9975(67)90039-4. [DOI] [PubMed] [Google Scholar]
  12. Marsh R. F., Semancik J. S., Medappa K. C., Hanson R. P., Rueckert R. R. Scrapie and transmissible mink encephalopathy: search for infectious nucleic acid. J Virol. 1974 May;13(5):993–996. doi: 10.1128/jvi.13.5.993-996.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nomura M., Erdmann V. A. Reconstitution of 50S ribosomal subunits from dissociated molecular components. Nature. 1970 Nov 21;228(5273):744–748. doi: 10.1038/228744a0. [DOI] [PubMed] [Google Scholar]
  14. Sogo J. M., Koller T., Diener T. O. Potato spindle tuber viroid. X. Visualization and size determination by electron microscopy. Virology. 1973 Sep;55(1):70–80. doi: 10.1016/s0042-6822(73)81009-8. [DOI] [PubMed] [Google Scholar]

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