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. 1974 Nov;14(5):1142–1151. doi: 10.1128/jvi.14.5.1142-1151.1974

Fragment Maps of φX-174 Replicative DNA Produced by Restriction Enzymes from Haemophilus aphirophilus and Haemophilus influenzae H-I

Marie N Hayashi 1, Masaki Hayashi 1
PMCID: PMC355631  PMID: 4610178

Abstract

φX-174 replicative form (RF) DNA was cleaved by restriction enzymes from Haemophilus aphirophilus (Hap) and H. influenzae (H-I strain Osaka) (HinH). Five fragments were produced by Hap and eight by HinH. The positions of all of the Hap fragments and six of the HinH fragments were mapped on the genome by heteroduplex transfection and DNA-DNA hybridization methods. In addition, fragment sizes of φX-174 RF DNA and S13 RF DNA by Hap and HinH were compared. Considerable differences of the size of the fragments produced from these closely related phage genomes were observed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benbow R. M., Hutchison C. A., Fabricant J. D., Sinsheimer R. L. Genetic Map of Bacteriophage phiX174. J Virol. 1971 May;7(5):549–558. doi: 10.1128/jvi.7.5.549-558.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bishop D. H., Claybrook J. R., Spiegelman S. Electrophoretic separation of viral nucleic acids on polyacrylamide gels. J Mol Biol. 1967 Jun 28;26(3):373–387. doi: 10.1016/0022-2836(67)90310-5. [DOI] [PubMed] [Google Scholar]
  3. Danna K. J., Sack G. H., Jr, Nathans D. Studies of simian virus 40 DNA. VII. A cleavage map of the SV40 genome. J Mol Biol. 1973 Aug 5;78(2):363–376. doi: 10.1016/0022-2836(73)90122-8. [DOI] [PubMed] [Google Scholar]
  4. Edgell M. H., Hutchison C. A., 3rd, Sclair M. Specific endonuclease R fragments of bacteriophage phiX174 deoxyribonucleic acid. J Virol. 1972 Apr;9(4):574–582. doi: 10.1128/jvi.9.4.574-582.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Funk F., Person S. Cytosine to thymine transitions from decay of cytosine-5-3H in bacteriophage S13. Science. 1969 Dec 26;166(3913):1629–1631. doi: 10.1126/science.166.3913.1629. [DOI] [PubMed] [Google Scholar]
  6. GUTHRIE G. D., SINSHEIMER R. L. Observations on the infection of bacterial protoplasts with the deoxyribonucleic acid of bacteriophage phi X174. Biochim Biophys Acta. 1963 Jun 25;72:290–297. [PubMed] [Google Scholar]
  7. Godson G. N. DNA heteroduplex analysis of the relation between bacteriophages phiX174 and S.13. J Mol Biol. 1973 Jul 15;77(4):467–477. doi: 10.1016/0022-2836(73)90216-7. [DOI] [PubMed] [Google Scholar]
  8. Hayashi M. N., Hayashi M. Isolation of phi X174 specific messenger ribonucleic acids in vivo and identification of their 5' terminal nucleotides. J Virol. 1972 Feb;9(2):207–215. doi: 10.1128/jvi.9.2.207-215.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hayashi Y., Hayashi M. Template activities of the phi X-174 replicative allomorphic deoxyribonucleic acids. Biochemistry. 1971 Nov;10(23):4212–4218. doi: 10.1021/bi00799a009. [DOI] [PubMed] [Google Scholar]
  10. Hedgpeth J., Goodman H. M., Boyer H. W. DNA nucleotide sequence restricted by the RI endonuclease. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3448–3452. doi: 10.1073/pnas.69.11.3448. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jeng Y., Gelfand D., Hayashi M., Shleser R., Tessman E. S. The eight genes of bacteriophages phi X174 and S13 and comparison of the phage-specified proteins. J Mol Biol. 1970 Apr 28;49(2):521–526. doi: 10.1016/0022-2836(70)90262-7. [DOI] [PubMed] [Google Scholar]
  12. Johnson P. H., Lee A. S., Sinsheimer R. L. Production of specific fragments of phi X174 replicative form DNA by a restriction enzyme from Haemophilus parainfluenzae, endonuclease HP. J Virol. 1973 Apr;11(4):596–599. doi: 10.1128/jvi.11.4.596-599.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kelly T. J., Jr, Smith H. O. A restriction enzyme from Hemophilus influenzae. II. J Mol Biol. 1970 Jul 28;51(2):393–409. doi: 10.1016/0022-2836(70)90150-6. [DOI] [PubMed] [Google Scholar]
  14. Linney E. A., Hayashi M. N., Hayashi M. Gene A of X174. 1. Isolation and identification of its products. Virology. 1972 Nov;50(2):381–387. doi: 10.1016/0042-6822(72)90389-3. [DOI] [PubMed] [Google Scholar]
  15. Middleton J. H., Edgell M. H., Hutchison C. A., 3rd Specific fragments of phi X174 deoxyribonucleic acid produced by a restriction enzyme from Haemophilus aegyptius, endonuclease Z. J Virol. 1972 Jul;10(1):42–50. doi: 10.1128/jvi.10.1.42-50.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sclair M., Edgell M. H., Hutchinson C. A., 3rd Mapping of new Escherichia coli K and 15 restriction sites on specific fragments of bacteriophage phi X174. J Virol. 1973 Mar;11(3):378–385. doi: 10.1128/jvi.11.3.378-385.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sugisaki H., Takanami M. DNA sequence restricted by restriction endonuclease AP from Haemophilus aphirophilus. Nat New Biol. 1973 Dec 5;246(153):138–140. doi: 10.1038/newbio246138a0. [DOI] [PubMed] [Google Scholar]
  18. TESSMAN E. S., SHLESER R. Genetic recombination between phages S13 and phi-X174. Virology. 1963 Feb;19:239–240. doi: 10.1016/0042-6822(63)90015-1. [DOI] [PubMed] [Google Scholar]
  19. Takanami M., Kojo H. Cleavage site specificity of an endonuclease prepared from Heamophilus influenzae strain H-I. FEBS Lett. 1973 Feb 1;29(3):267–270. doi: 10.1016/0014-5793(73)80035-3. [DOI] [PubMed] [Google Scholar]
  20. ZAHLER S. A. Some biological properties of bacteriophages S13 and theta X-174. J Bacteriol. 1958 Mar;75(3):310–315. doi: 10.1128/jb.75.3.310-315.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]

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