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. 2012 Nov 14;5:636. doi: 10.1186/1756-0500-5-636

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Copyright ©2012 Khan et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Active and total TGF-β levels in sham and two weeks post-myocardial infarction (MI) mice left ventricular myocardium measured by ELISA and bioassay.A: Active TGF-β levels and B: Total TGF-β levels in sham and two weeks post-MI heart homogenate. For both sham and MI, bioassay revealed not only significantly higher TGF-β levels than ELISA (p < 0.05), but also showed significant increase of both TGF-β levels in MI compared with sham (p < 0.05), whereas ELISA failed to show significant increase in MI compared with sham. Sample size: n = 6. ELISA=enzyme-linked immunosorbent assay, MI=myocardial infarction, TGF-β=transforming growth factor-β.