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. Author manuscript; available in PMC: 2013 Oct 16.
Published in final edited form as: Biochemistry. 2012 Oct 4;51(41):8071–8084. doi: 10.1021/bi3006658

Table 2.

Analysis of Mössbauer resonances as a percentage of total Fe during NifS-mediated Fe-S cluster assembly on NifIscA as a function of reaction time. Values in parentheses are estimated uncertainties for the last significant digits.

Sample reaction time (min) FeIICys4 FeII-3.5 FeII-4.1 [2Fe-2S]2+ [4Fe-4S]2+
NifIscA (−) (control) 2 51 (5) 36 (4) 11 (2) – 2 (2)
NifIscA (+) 2 56 (5) 28 (3) 14 (2) – 2 (2)
NifIscA (−) (control) 20 36 (4) 41 (4) 18 (2) – 5 (2)
NifIscA (+) 20 39 (4) 27 (3) 14 (2) 9 (2) 11 (2)
NifIscA (−) (control) 60 34 (4) 44 (5) 16 (2) – 6 (2)
NifIscA (+) 60 24 (3) 21 (3) 11 (2) 3 (2) 41 (4)