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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: J Invest Dermatol. 2012 Oct 25;133(3):827–830. doi: 10.1038/jid.2012.379

Figure 2. Exome sequencing reveals somatic HRAS and KRAS mutations in nevus sebaceus tissue.

Figure 2

(a) HRAS and KRAS mutation annotation, including genomic position, nucleotide change, protein consequence, and number of reference and non-reference reads obtained from paired sequencing of tissue and blood in 5 independent, unrelated nevus sebaceus cases. Significance of the mutant allele frequency difference between tissue and blood DNA was calculated with a one-tailed Fisher’s exact test. When corrected for multiple testing, 2.4×10−6 is the threshold for genome wide significance. In each case, HRAS and KRAS mutations showed the lowest P-value. (b) Alignment of the N-termini of HRAS and KRAS reveals identical residues through position 94, with an overall 95% identity and 99% similarity. The first 50 amino acids are shown for the wild-type and each mutant protein, with mutant residues indicated in red.