Table 3.
Counts (and corresponding percentages) for suture patency in Fgfr2cC342Y/+ Crouzon syndrome mice and unaffected littermates at P0.
| SUTUREa | CORONAL | ZYGOMATIC- MAXILLARY |
PREMAXILLARY- MAXILLARY |
INTER- PREMAXILLARYb |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Open | Partial | Fused | TOTAL | Open | Fused | TOTAL | Open | TOTAL | 233 | 333 | TOTAL | |
| Fgfr2c+/+ | 20 (65%) | 10 (32%) | 1c (3%) | 31 | 31 (100%) | 0 (0%) | 31 | 31 (100%) | 31 | 9 (23%) | 30 (77%) | 39 |
| Fgfr2cC342Y/+ | 1 (4%) | 4 (14%) | 23 (82%) | 28 | 26 (93%) | 2 (7%) | 28 | 28 (100%) | 28 | 16 (47%) | 18 (53%) | 34 |
| Totald | 21 | 14 | 24 | 59 | 57 | 2 | 59 | 59 | 59 | 14 | 48 | 73 |
| SUTURE | INTER-PALATINE | MAXILLARY-PALATINE | FRONTO-MAXILLARY |
FRONTO- PREMAXILLARY |
FRONTO-NASAL | |||||||
| Open | Partial | TOTAL | Open | Fused | TOTAL | Open | TOTAL | Open | TOTAL | Open | TOTAL | |
| Fgfr2c+/+ | 30 (77%) | 9 (23%) | 39 | 26 (67%) | 13 (33%) | 39 | 39 (100%) | 39 | 39 (100%) | 39 | 39 (100%) | 39 |
| Fgfr2cC342Y/+ | 30 (88%) | 4 (12%) | 34 | 28 (82%) | 6 (18%) | 34 | 34 (100%) | 34 | 34 (100%) | 34 | 34 (100%) | 34 |
| Totald | 60 | 13 | 73 | 54 | 19 | 73 | 73 | 73 | 73 | 73 | 73 | 73 |
For each group, qualitative scores on suture patency (open, partial, fused) as visualized on µCT images are provided. Open: No closure or overlap visualized at either side of the suture (both sides scored as open). Partial: Partial fusion or “bridging” apparent. Fused: almost no open areas visualized along sutural margins. For a more complete definition of patency states see Materials and Methods.
The inter-premaxilla suture was divided into thirds: the most anterior third, medial third, and most posterior third. Each section was scored on the degree of fusion: a score of 1 indicated the suture was completely patent; 2 indicated partial fusion; 3 indicated complete fusion. The sutures were scored from anterior to posterior. A score of 123 indicates the anterior third is open, the medial third is partially fused, and posterior third is fused; 233 refers to inter-premaxillary sutures that remained patent anteriorly but were otherwise not patent; 333 refers to inter-premaxillary sutures that showed no visible patency by inspection. See Supplementary methods for details.
This Fgfr2c+/+ mouse displayed complete fusion of the left coronal suture and partial fusion of the right coronal suture.
Totals vary for suture counts in cases where a mouse was damaged during processing and specific suture(s) could not be scored for patency reliably.