Table 1.
Primer sets for the PCR amplification of archaeal genes that encode key enzymes of nitrogen metabolism.
| Process in N cycle | Key enzyme | Target gene | Primer name | Primer sequence | Reference |
|---|---|---|---|---|---|
| NH4 + → NO2 − | Ammonia monooxygenase (E.C. 1.14.99.39.) |
amoA | arc-Amo-f arc-Amo-r |
5′-STAATGGTCTGGCTTAGACG-3′ 5′-GCGGCCATCCATCTGTATGT-3′ |
[18] |
| NO2 − → NH4 + | Ferredoxin nitrite reductase (E.C. 1.7.7.1.) |
nirA | arc-NirA-f arc-NirA-r |
5′-AAYMTSCCNCGGAAGTKSAA-3′ 5′-AGAACTCCBTRCCSGTRCAS-3′ |
This study |
| NO2 − → NH4 + | Ammonifying nitrite reductase (E.C. 1.7.1.4.) |
nirB | arc-NirB-f arc-NirB-r |
5′-ATGCTGAGCCATTAYATAGC-3′ 5′-CCGTTGTACTCGGCRCAGTC-3′ |
This study |
| N2O → N2 | Nitrous oxide reductase (E.C. 1.7.2.4.) |
nosZ | arc-Nos-f arc-Nos-r |
5′-TGGGGHGACTMYCAYCAYCC-3′ 5′-AKGTGKCCRRSGTTGTAGTK-3′ |
This study |
| N2 → NH4 + | Nitrogenase reductase (E.C. 1.18.6.1.) |
nifH | arc-Nif-f arc-Nif-r |
5′-TAYGGAAARGGNGGNATYGG-3′ 5′-CCNCCRCAGACRACRTCNCC-3′ |
This study |
| NO3 − → NO2 − | Dissimilatory nitrate reductase (E.C.1.7.99.4.) |
napA, narG | arc-Nred-f arc-Nred-r |
5′-CGACTGGTAYKCVGAYCTHC-3′ 5′-GTCRGYGTKRWACCAGTSGK-3′ |
This study |