Figure 2.

Cx cr5^−/− mice lack CD8⁺CXCR5⁺ cells. Spleen cells were isolated from C57BL/6 WT mice 7 days after genital infection with C. muridarum and stained with CD3, CD4 or CD8, CXCR5, and markers of T cells; TCRγδ, TCRβ, CD8β or NKT cells; CD1d-tetramer. (a) The dotplot shows the percentage of CD8 and CXCR5 of CD3⁺ cells from Cxcr5^−/− and WT mice. (b) The percent of CD4 or CD8 populations from groups of 6 mice were compared between Cxcr5^−/− and WT mice by Student's t-test. The ∗ indicates P < 0.01. (c) The percentage of CD4 or CD8 cells expressing markers of T cells; TCRγ, TCRβ, CD8β or NKT cells; CD1d-tetramer.