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. Author manuscript; available in PMC: 2013 Jan 27.
Published in final edited form as: Insect Mol Biol. 2011 Jun 24;20(5):587–598. doi: 10.1111/j.1365-2583.2011.01089.x

Figure 2.

Figure 2

Northern blot analyses of EGFP transcription in female midguts of the mariner Mos1 transformed lines (G4–5) Carb/egfp52, 112, 169, 54, and (G19) 105. Samples were prepared from midguts harvested after sugarfeeding (SF) or 4–96 h pbm. The 10 carcasses of Carb/egfp105 mosquitoes were without heads. The hybridization pattern of Carb/egfp157, 188, and 191 (not shown) resembled those of Carb/egfp54 and 169. Total RNA was extracted from pools of 25 midguts per time point. Blots were hybridized with random primed 32P-dCTP- labeled DNA probes or in vitro-transcribed 32P-dUTP-labeled probes corresponding to the EGFP gene at 48°C. Ethidium bromide stained agarose gels are shown as loading controls.