Skip to main content
. Author manuscript; available in PMC: 2013 Jan 27.
Published in final edited form as: Insect Mol Biol. 2011 Jun 24;20(5):587–598. doi: 10.1111/j.1365-2583.2011.01089.x

Figure 6.

Figure 6

ΦC31-mediated recombination of line attP26 with the attB/DsRed2-PUb/v5B2/ svA donor plasmid to constitutively express the Flock House virus B2 suppressor of RNA interference in Ae. aegypti. (A) Diagram of the donor plasmid integration pattern following recombination between the attP site of line attP26 and the attB site of the donor. Grey bar indicates the part of the transgene that originates from the donor plasmid. (B) Northern blot showing v5B2 expression over time in various tissues of recombinant P61 mosquitoes. Lanes: (1) docking strain attP26 (negative control); (2) P61 larvae; (3) P61 females - midguts, 1 day post-emergence (pe); (4) carcasses, 1 day pe; (5) midguts, 3 days pe; (6) carcasses, 3 days pe; (7) midguts, 5 days pe; (8) carcasses, 5 days pe; (9) midguts, 7 days pe; (10) carcasses, 7 days pe; (11) midguts of bloodfed females, 7 days post-bloodmeal (pbm); (12) carcasses of bloodfed females, 7 days pbm; (13) midguts of bloodfed females, 14 days pbm; (14) carcasses of bloodfed females, 14 days pbm; (15) P61 males - 1 day pe; (16) 3 days pe; (17) 5 day pe; (18) 7 days pe.