Table 1.
Transformation data of Aedes aegypti lines (HWE strain) using mariner Mos1 or piggyBac TEs as insertion vectors or ΦC31 for site-directed recombination
| Mos1-Carb/egfp | attP26-attB, ‘WT’ ΦC31 |
attP26-attB, ‘mut.’ ΦC31 |
piggyBac- attP site |
attP26- attB/Carb/egfp |
|
|---|---|---|---|---|---|
| No. of embryos injected | 1140 | 297 | 557 | 800 | 1340 |
| No. of G0 survivors | 193 | 89 | 181 | 82 | 120 |
| No. of G1 families (f) or pools (p)1 | 116f | 13f, 3p | 22f, 5p | 41f | 27p |
| No. of G2 transgenic families or pools | 9f | 1f, 1p | 2f, 2p | 2f | 1p |
| estimated TF or RF2 | 9.3 % | 4.5 % | 4.4 % | 4.9 % | 1.7 % |
1
family: 1 transgenic male × 20 HWE females or 4–5 transgenic males × 40–50 HWE females; pool sizes ranged from 4 transgenic females × 4 HWE males to 19 transgenic females × 16 HWE males.
2
estimated transformation or recombination frequency: number of independent integration events divided by number of surviving G0 mosquitoes × 0.5 (estimated average fertility rate per individual).