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. 2013 Jan 4;3(1):e99. doi: 10.1038/bcj.2012.44

Figure 4.

Figure 4

Restoration of p15Ink4b expression in bone marrow cells of Ink4bKO mice restores erythoid/myeloid progenitor cell balance. (a) Infection efficiencies of Lin− bone marrow cells from Ink4bKO mice transduced with the pLVX-PTuner-Green vector containing murine p15Ink4b cDNA (p15LentiX) or empty vector (LentiX). (b) Representative pictures of BFU-E colonies obtained from plating p15Ink4b-transduced and in vitro-differentiated cells (S17 monolayer, SCF, SH) in M3436 methylcellulose medium. (c) Number of BFU-E colonies obtained by plating 10 000 of transduced and in vitro-differentiated cells in M3436 methylcellulose medium. (d) Number of myeloid colonies (CFU-GM, CFU-G and CFU-M) obtained by plating 1000 of the same cells as in (c) in M3534 medium. Following overnight infection, cells were differentiated for 60 h on S17 stromal cells in the presence of SH (n=9). Non-transduced wild-type (WT) and Ink4bKO cells were carried through the same experimental procedure as transduced cells for direct comparison within the experiment.